Journal: Eukaryotic Cell

Article Title: Regulation of Cryptococcus neoformans Capsule Size Is Mediated at the Polymer Level

doi: 10.1128/ec.00437-07

Figure Lengend Snippet: FIG. 1. Electromobility of shed GXM from JEC21 cells changes over time in rich medium. All samples were heated (15 min at 70°C) to denature enzymes, centrifuged (16,000 g for 3 min) to separate supernatant and cells, and stored at 4°C. Samples (15 l) of culture supernatants were mixed with 6 DNA loading dye (4), loaded on a 0.6% certified megabase agarose (Bio-Rad) gel, and subjected to elec- trophoresis (15 h at 25 V) in 0.5 TBE (44.5 mM Tris base, 44.5 mM boric acid, 1 mM EDTA, pH 8.3). Samples containing more GXM (later time points) were diluted in distilled water based on a pilot gel, as sample normalization based on enzyme-linked immunosorbent as- say determination of GXM concentration (23) did not yield equal blot intensity. (This may reflect changes over time in GXM antibody reac- tivity [7, 11, 18] and/or transfer efficiency.) The gel was transferred onto a positively charged nylon membrane and immunoblotted with 1 g/ml anti-GXM antibody 3C2 as described in the text. Initial sample heating and dilution in distilled water did not alter electrophoretic migration (data not shown). St, starter culture (at 1.6 107 cells/ml); arrowhead, well position; arrow, direction of migration; other num- bers, sampling time (hours) after the start of the experiment.

Article Snippet: Samples (15 l) of culture supernatants were mixed with 6 DNA loading dye (4), loaded on a 0.6% certified megabase agarose (Bio-Rad) gel, and subjected to electrophoresis (15 h at 25 V) in 0.5 TBE (44.5 mM Tris base, 44.5 mM boric acid, 1 mM EDTA, pH 8.3).

Techniques: Concentration Assay, Membrane, Migration, Sampling

Journal: Cell

Article Title: Microglia Require CD4 T Cells to Complete the Fetal-to-Adult Transition

doi: 10.1016/j.cell.2020.06.026

Figure Lengend Snippet:

Article Snippet: 96.96 DNA Binding Dye Sample/Loading Kit—10 IFCs, with Control Line Fluid , Fluidigm , Cat#BMK-M10- 96.96-EG.

Techniques: Labeling, Plasmid Preparation, Purification, Recombinant, Binding Assay, Cell Isolation, Conjugation Assay, Sequencing, Transgenic Assay, Knock-Out, Software